About 100 years later c435ad, Socrates Scholasticus wrote this:
The fasts before Easter will be found to be differently observed among different people. Those at Rome fast three successive weeks before Easter, excepting Saturdays and Sundays.784
784 Baronius (Ann. 57 and 391 a.d.) finds two mistakes here: first, in the assertion that the Romans fasted three weeks only before Easter, and second, in the assertion that during those three weeks Saturdays were excepted. Cf. also Ceillier, Hist. des Auteurs Sacrés et Ecclesiast. Vol. VIII. p. 523, 524. Valesius, however, quotes Pope Leo (fourth sermon on the Lent Fast) and Venerable Beda to prove that Socrates assertion concerning the exception of Saturday may be defended. See Quesnell, de Jejunio Sabbati; Bingham, Origin. Eccl. XXI. I. 14; also Beveridge, de Jejunio Quadragesimali.
Those in Illyrica and all over Greece and Alexandria observe a fast of six weeks, which they term The forty days fast.785785 Τεσσαρακοστή = Lent; the Latin equivalent is, of course, Quadragesima. Others commencing their fast from the seventh week before Easter, and fasting three five days only, and that at intervals, yet call that time The forty days fast. It is indeed surprising to me that thus differing in the number of days, they should both give it one common appellation; but
some assign one reason for it, and others another, according to their several fancies. One can see also a disagreement about the manner of abstinence from food, as well as about the number of days. Some wholly abstain from things that have life: others feed on fish only of all living creatures: many together with fish, eat fowl also, saying that according to Moses,786 these were likewise made out of the waters. Some abstain from eggs, and all kinds of fruits: others partake of dry bread only; still others eat not even this: while others having fasted till the ninth hour, afterwards take any sort of food without distinction. And among various nations there are other usages, for which innumerable reasons are assigned. Since however no one can produce a written command as an authority,
it is evident that the apostles left each one to his own free will in the matter, to the end that each might perform what is good not by constraint or necessity. Such is the difference in the churches on the subject of fasts.
NPNF2-02. Socrates and Sozomenus Ecclesiastical Histories - Christian Classics Ethereal Library
So again, it's clear there were fasting periods before pascha. This fast controversy was also referenced as early as 155ad with Polycarp visit Anicetus.
But still nothing at this stage regarding an ash Wednesday.