It depends on what you mean by "knowledge".
If you're talking about "knowledge" in the technical, epistemological sense then knowledge would be quite unnecessary. If the only things that are possible are things that you
know exist then the whole notion of possibility is irrelevant. In that scenario, nothing at all is
possible. There are only actualities and impossibilities. But real life is much more complicated than this. There are legitimate possibilities. I don't know what I'm going to have for dinner, but it's possible that I might have a hamburger.
If you mean that you need to have some
experience of a thing in order to determine whether or not its existence is possible then this is equally limited. We have no experience of extra terrestrial life, but we would not usually thereby say that extra terrestrial life is impossible. And we would not say that we cannot know whether or not extra terrestrial life is possible.
If you mean that you need to be able to
imagine a thing or circumstance in order to determine whether or not it's possible, that's fair. But we can easily imagine something like a unicorn. Just take a horse and put a horn on it. We can also easily imagine some other place in the universe where a unicorn might exist. So I don't think it's too difficult to say that it's possible for a unicorn to exist somewhere in some possible world. It's possible that evolution could have worked out in such a way to develop unicorns on our planet, although this did not actually happen. Even though it didn't actually happen, it's still possible.
If you're still confused about the concept of possibility, you should read up on
modal logic.
No. Many things are quite impossible. For example a square circle is impossible. It could not exist in any possible world. Likewise, it's impossible that the number "2" could not exist. It's also impossible that 2+2 could ever equal 5. Etc.