That was part of the explanation from my previous post. I provided the links to the papers. I hate that some sites make you pay for the paper but if you can find the email address of one of the researchers they'll be more than happy to send a copy to you!
Warm-Blooded Isochore Structure in Nile Crocodile and Turtle
Sandrine Hughes,* Dominique Zelus, and Dominique Mouchiroud*
Abstract
The genomes of warm-blooded vertebrates are characterized by a strong heterogeneity in base composition, with
GC-rich and GC-poor isochores. The GC content of sequences, especially in third codon positions, is highly correlated
with that of the isochore they are embedded in. In amphibian and fish genomes, GC-rich isochores are
nearly absent. Thus, it has been proposed that the GC increase in a part of mammalian and avian genomes represents
an adaptation to homeothermy. To test this selective hypothesis, we sequenced marker protein genes in two coldblooded
vertebrates, the Nile crocodile
Crocodylus niloticus (10 genes) and the red-eared slider Trachemys scripta
elegans (6 genes). The analysis of base composition in third codon position of this original data set shows that the
Nile crocodile and the turtle also exhibit GC-rich isochores, which rules out the homeothermy hypothesis. Instead,
we propose that the GC increase results from a mutational bias that took place earlier than the adaptation to
homeothermy in birds and before the turtle/crocodile divergence. Surprisingly, the isochore structure appears very
similar between the red-eared slider and the Nile crocodile than between the chicken and the Nile crocodile. This
point questions the phylogenetic position of turtles as a basal lineage of extant reptiles. We also observed a regular
molecular clock in the Archosauria, which enables us, by using a more extended data set, to confirm Kumar and
Hedgess dating of the bird-crocodile split.
Discussion
As expected, phylogenetic analysis of the 10 presently
sequenced genes from the Nile crocodile strongly
supports the Archosauria, which groups birds and crocodilians
in a sister group with bootstrap values higher
than 85%, except for vdr (59%) and pk (71%). As a
whole, these data definitively rule out the Haemothermia
hypothesis, which groups birds and mammals in
a sister group (Hedges, Moberg, and Maxon 1990).
For the divergence time between birds and crocodilians,
a recent work has proposed a mean value of 222
6 40 Myr (Kumar and Hedges 1998), in agreement with
the 240-Myr value suggested by fossil analyses (Benton
1990). The new divergence time (215 Myr) that we estimated
on 10 concatenated nuclear coding genes (i.e.,
2.5 more genes than in previous studies) is in accordance
with Kumars findings. Analysis of the avian/
crocodilian split, performed on the complete mitochondrial
genome of Alligator mississippiensis, gives a divergence
time of 254 Myr (Janke and Arnason 1997),
β-Keratins in crocodiles reveal amino acid homology with avian keratins
Changjiang Ye1, Xiaobing Wu1, Peng Yan1 and George Amato2
Abstract
The DNA sequences encoding β-keratin have been obtained from Marsh Mugger (Crocodylus palustris) and Orinoco Crocodiles (Crocodylus intermedius). Through the deduced amino acid sequence, these proteins are rich in glycine, proline and serine. The central region of the proteins are composed of two beta-folded regions and show a high degree of identity with β-keratins of aves and squamates. This central part is thought to be the site of polymerization to build the framework of β-keratin filaments. It is believed that the β-keratins in reptiles and birds share a common ancestry. Near the C-terminal, these β-keratins contain a peptide rich in glycine-X and glycine-X-X, and the distinctive feature of the region is some 12-amino acid repeats, which are similar to the 13-amino acid repeats in chick scale keratin but absent from avian feather keratin. From our phylogenetic analysis, the β-keratins in crocodile have a closer relationship with avian keratins than the other keratins in reptiles.
So here again we have two separate studies that still show the genetic relatedness of crocodiles and birds.
Again, why would God give crocodiles and birds such a close relationship if evolution is false?