Yes- and "for my sake" is key to note there. To lose our life in a manner so that we may find it (i.e. with a faith God will accept) means that we die to self to follow the Word of God with our whole heart, soul, strength, and mind. That requires serving no other masters and subjecting our thoughts, emotions, goals, values, principles, and opinions to God's Word; that requires intellectual effort to be put forth and intellectual honesty to be exercised when evaluating God's Word; that requires denying our own desires and assumed rights that would lead us to go contrary to the Word; that requires yielding up and offering our powers, our ambitions, our self-esteem, our family, our possessions, and our comfort to follow the Word faithfully. There is a lot to lose, but exceedingly more to gain!
Philippians 2:5-16: "5 Let this mind be in you, which was also in Christ Jesus: 6 Who, being in the form of God, thought it not robbery to be equal with God: 7 But made himself of no reputation, and took upon him the form of a servant, and was made in the likeness of men: 8 And being found in fashion as a man, he humbled himself, and became obedient unto death, even the death of the cross. 9 Wherefore God also hath highly exalted him, and given him a name which is above every name: 10 That at the name of Jesus every knee should bow, of things in heaven, and things in earth, and things under the earth; 11 And that every tongue should confess that Jesus Christ is Lord, to the glory of God the Father. 12 Wherefore, my beloved, as ye have always obeyed, not as in my presence only, but now much more in my absence, work out your own salvation with fear and trembling. 13 For it is God which worketh in you both to will and to do of his good pleasure. 14 Do all things without murmurings and disputings: 15 That ye may be blameless and harmless, the sons of God, without rebuke, in the midst of a crooked and perverse nation, among whom ye shine as lights in the world; 16 Holding forth the word of life; that I may rejoice in the day of Christ, that I have not run in vain, neither laboured in vain."