... or, how come scientists think there are 23 pairs of chromosomes?
It's interesting to see how the story has been considered in popular understanding, compared to what actually happened.
When I was in high school and in college, one of the best known scientific facts was that the human cell contained 48 chromosomes (24 pairs. My faith in science as a whole was seriously shaken when during my Junior year in the university it was suddenly discovered that the human cell only contained 46 chromosomes (23 pairs).
Now I know that you couldn't have been in your junior year in university any time before 1921! It was in that year that Theophilus S. Painter published his paper (available
here) detailing the "fact" that humans have 48 chromosomes. However, people had already been counting chromosomes since the 1890s. But of course you should be asking: what, exactly,
are chromosomes? They're essentially bundles of genetic material at the highest stage of organization. However, you can't just open up a cell at any time and see chromosomes: they only bunch up in their iconic "X" shapes during mitosis and meiosis, when you can stain them with certain chemicals. (This will be important later.)
Anyways. In the 1890s, the first few counts made were actually
lower than they should have been. Much lower: you'd expect 46, but in fact in the five early papers published, all but one counted 24! (The odd one out had 47 in ovaries and 48 in testes; surprisingly close.) So when Painter published his figure of 48 in 1921, again, it's a surprise he even got close. (More on that later.) In fact, part of his original paper reads:
"In my own materials the counts range from 45 to 48 apparent chromosomes, although in the clearest plates so far studied only 46 chromosomes have been found."
This was a very simple fact, something anyone with a microscope could count. yet because some authority figure said there were 24 pairs of chromosomes in the human genome, everyone simply counted and recounted until they got 24 pairs, and then said, ah, I finally got it right!
Oh no, it's not a simple fact at all. Above I quoted Painter himself counting anywhere between 45 and 48 chromosomes. Look at one of the diagrams he drew of those chromosomes. Is it any wonder why he couldn't get a fix on it?
It's a wonder he even got anywhere
near the correct figure!
What made chromosome counting so difficult? Firstly, it was difficult to find fresh material. Remember that chromosomes only bunch up for counting when a cell is dividing. (How would scientists know? Manipulate cells into different states chemically, e.g. by starving them or feeding them extra nutrients, and then stain them to see what's there and what's not.) It's difficult to get samples of rapidly dividing human cells for study; the main source were reproductive organs (hence the reference to ovaries and testes earlier). Cytologists "literally waited at the foot of the gallows in order to fix the testis of an executed criminal immediately after death before the chromosomes could clump." Also, their method of preparing cells (paraffin sectioning) couldn't separate chromosomes out.
Over the next few decades more techniques appeared for cytologists: hypotonic shock could cause a cell's chromosomes to spread out before slide preparation, and colchicine could arrest cells so that more of them were stuck in the metaphase where chromosomes were bunched up.
Everyone, that is, until a single japanese student counted and recounted until he was certain that the cell he was observing only had 23 pairs. Itwas immediately announced that it had been discovered that some human cells only contained 23 pairs of chromosomes. This freed everyone to count with an open mind, and within two months it was announced that no human cells contained 24 pairs of chromosomes. They only contained 23 pairs.
He wasn't single, and he wasn't Japanese! Though it's no surprise that people might mistake him for one:
Joe Hin Tjio was born of Chinese ancestry in Java in 1919, which would make him 37 when he published the 1956 paper describing the fact that humans have 46 chromosomes. (Hardly a poster boy for "young researcher overturns established dogma" now, is he?) Granted, he was
captured by the Japs when they invaded Southeast Asia during WWII.
And it wasn't "counting and recounting" that got him the number 46. In fact, the number had been reported earlier. A fellow student at the instute with Joe recalls being told by the director: "earlier that year [1955] Doctors Eva and Yngve Melander working on normal human fetal cells had problems with their chromosome preparation as they could only find cells with incomplete chromosome plates, the maximum number being 46." Which just goes to show that scientists don't always cover up anomalies! But anyways, it wasn't "counting and recounting" that got him 46. It was:
You don't have to count and recount with an image like that! It's obvious that this new picture is far more reliable than the old one on which the count of 48 chromosomes was based.
So we know that supposed scientific facts are not as reliable as the cult of scientism would have us to believe.
No, we know that old, unreliable "scientific facts" can always be uprooted by new, more reliable scientific facts.
Cool isn't it?
All information obtained from this article:
The chromosome number in humans: A brief history (warning, might require subscription)