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BOMBSHELL: WHO Coronavirus PCR Test Primer Sequence is Found in All Human DNA
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<blockquote data-quote="Tanj" data-source="post: 75279600" data-attributes="member: 396712"><p>Once again, only for the second primer. There are no exact matches for the first (from the human genome). You need both primers to bind to do the geometric amplification during the synthesis steps. Even if some of one of the primers is off binding somewhere else, it's not going to do any amplification, and after a few cycles the true product will be so overwhelmingly abundant the off target binds will be irrelevant.</p><p></p><p></p><p></p><p>I don't know what that means,.</p><p></p><p></p><p></p><p>It doesn't matter. As I said, this procedure requires 100% match to primer 1 and primer 2 precisely 108 bp apart and then a 100% match to primer 3 in that 108 bp section. The presence of other RNA species is irrelevant, that's kind of the whole point of PCR, to amplify up a specific piece of NA against a large heterogeneous background.</p></blockquote><p></p>
[QUOTE="Tanj, post: 75279600, member: 396712"] Once again, only for the second primer. There are no exact matches for the first (from the human genome). You need both primers to bind to do the geometric amplification during the synthesis steps. Even if some of one of the primers is off binding somewhere else, it's not going to do any amplification, and after a few cycles the true product will be so overwhelmingly abundant the off target binds will be irrelevant. I don't know what that means,. It doesn't matter. As I said, this procedure requires 100% match to primer 1 and primer 2 precisely 108 bp apart and then a 100% match to primer 3 in that 108 bp section. The presence of other RNA species is irrelevant, that's kind of the whole point of PCR, to amplify up a specific piece of NA against a large heterogeneous background. [/QUOTE]
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BOMBSHELL: WHO Coronavirus PCR Test Primer Sequence is Found in All Human DNA
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